Tissue engineering and regenerative medicine uses a combination of cells, scaffolding and bioreactive factors to treat a variety of pathological conditions and has become a treatment option for many adult diseases. In this book, the authors present current research from across the globe, in the study of the fundamentals, techniques and applications of tissue engineering.
Tissue engineering integrates knowledge and tools from biological sciences and engineering for tissue regeneration. A challenge for tissue engineering is to identify appropriate cell sources. The recent advancement of stem cell biology provides enormous opportunities to engineer stem cells for tissue engineering. The impact of stem cell technology on tissue engineering will be revolutionary.
This book covers state-of-the-art knowledge on the potential of stem cells for the regeneration of a wide range of tissues and organs, including cardiovascular, musculoskeletal, neurological and skin tissues. The technology platforms for studying and engineering stem cells, such as hydrogel and biomaterials development, microfluidics system and microscale patterning, are also illustrated. Regulatory challenges and quality control for clinical translation are also detailed. This book provides an comprehensive update on the advancement in the field of stem cells and regenerative medicine, and serves as a valuable resource for both researchers and students.
Readership: Life science scientists; biomedical researchers; cell biologists; academics, postgraduate students and advanced undergraduate students in cell biology, biochemistry and genetics; surgeons; clinicians; biotechnology and pharmaceutical industry professionals.
Stem Cells and Tissue Repair: Methods and Protocols presents in-depth methods for the three major approaches of rejuvenating an aging or sick body: latent regenerative capacity stimulated in a targeted way, replacement organs grown de novo and surgically implanted, and tissue surgically implanted and coaxed to integrate and restore problem areas. The first approach taps into the latent regenerative capacity of particular tissues, such as muscle, skin, fat, or bone marrow. The second approach induces and grows pluripotent stem cells, drives their differentiation along certain pathways such as germ layers, neural crest, liver, teeth or retina, and cultures organs such as pancreas and heart. While the third approach involves various engraftment techniques for neural tissue. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Stem Cells and Tissue Repair: Methods and Protocols provides state-of the-art methods descriptions and the references therein that will provide a suitable starting point for exploring the vast literature that has already developed for regenerative medicine.