Genetic Engineering of Mesenchymal Stem Cells

MSC (mesenchymal stem cells) have been reported to initiate revascularization after injury, to facilitate engraftment of blood-forming stem cells, and to reduce the incidence of graft-vs. host disease through their immune-suppressive qualities. Finally, bone marrow-derived MSC have been reported to home to areas of solid tumor revascularization, and thus may be used as delivery vehicles to target ablative agents into dividing tumor cells. Recently the characteristics of human MSC from adipose (fat) tissue have also been identified. The possibility of repairing tissues, speeding stem cell engraftment, and targeting solid tumors for specific killing, using MSC easily harvested from bone marrow, or better yet, from unwanted fat tissue, holds broad appeal, and is an intriguing possibility that could have dramatic effect on health care.

This book has information on how to isolate, grow, and characterize MSC from marrow and fat, and gives important insight into how these cells may be used for gene delivery and cellular therapies in the future. Updates on emerging clinical trials are given.

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Mesenchymal Stem Cell Therapy (Stem Cell Biology and Regenerative Medicine)

Over the past decade, significant efforts have been made to develop stem cell-based therapies for difficult to treat diseases. Multipotent mesenchymal stromal cells, also referred to as mesenchymal stem cells (MSCs), appear to hold great promise in regards to a regenerative cell-based therapy for the treatment of these diseases. Currently, more than 200 clinical trials are underway worldwide exploring the use of MSCs for the treatment of a wide range of disorders including bone, cartilage and tendon damage, myocardial infarction, graft-versus-host disease, Crohn’s disease, diabetes, multiple sclerosis, critical limb ischemia and many others.

 

MSCs were first identified by Friendenstein and colleagues as an adherent stromal cell population within the bone marrow with the ability to form clonogenic colonies in vitro.  In regards to the basic biology associated with MSCs, there has been tremendous progress towards understanding this cell population’s phenotype and function from a range of tissue sources.  Despite enormous progress and an overall increased understanding of MSCs at the molecular and cellular level, several critical questions remain to be answered in regards to the use of these cells in therapeutic applications.  Clinically, both autologous and allogenic approaches for the transplantation of MSCs are being explored.  Several of the processing steps needed for the clinical application of MSCs, including isolation from various tissues, scalable in vitro expansion, cell banking, dose preparation, quality control parameters, delivery methods and numerous others are being extensively studied.   Despite a significant number of ongoing clinical trials, none of the current therapeutic approaches have, at this point, become a standard of care treatment.  Although exceptionally promising, the clinical translation of MSC-based therapies is still a work in progress.

 

The extensive number of ongoing clinical trials is expected to provide a clearer path forward for the realization and implementation of MSCs in regenerative medicine. Towards this end, reviews of current clinical trial results and discussions of relevant topics association with the clinical application of MSCs are compiled in this book from some of the leading researchers in this exciting and rapidly advancing field.  Although not absolutely all-inclusive, we hope the chapters within this book can promote and enable a better understanding of the translation of MSCs from bench-to-bedside and inspire researchers to further explore this promising and quickly evolving field.

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Mesenchymal Stem Cells: Methods and Protocols (Methods in Molecular Biology)

This volume aims to outline the current status of the Mesenchymal Stem Cells(MSC) field in regenerative medicine and to propose clear and reproducible protocols to better define the identity, function and use of these cells that are today, more than ever, “under the spotlight”. Mesenchymal Stem Cells: Methods and Protocols, Second Edition is organized into four sections. The first guides the reader through a series of state-of-the-art reviews summarizing the use of MSC for the treatment of various diseases. The other three sections are a collection of methodological chapters covering several aspects: isolation and characterization of MSC; expansion of MSC for clinical use; production and characterization of the MSC secretome. Written in the highly successful Methods in Molecular Biology series format, the method chapters include introductions to their respective topics, complete lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting which will help the researcher to avoid known pitfalls.

Authoritative and cutting-edge, Mesenchymal Stem Cells: Methods and Protocols, Second Edition, aims to ensure successful results in the further study of this vital field.

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