This is the first experimental protocol book that covers the differentiation of bone marrow-derived stem cells (BMSCs) into specific cell types, targeted at the undergraduate and graduate student level. The 19 chapters deal with the differentiation methods using small molecules, cytokines and polymeric scaffolds.
BMSCs are pluripotential in that they not only act as myelo-regenerative and supportive cells, but can also differentiate into almost any kind of cells in our body. In addition, when implanted in vivo, they could help repair multiple tissues such as blood vessels, heart, liver and so on.
For the differentiation of BMSCs, many methods have been introduced to adjust their microenvironment (chemical and physical cues), including chemical induction methods using large or small molecules and pellet culture; mechanical stimulation induction methods using cyclic mechano-transduction or ultrasonication; cytokine-released method using scaffolds; and so on.
Readership: Undergraduate and graduate students and researchers in biomedical engineering, tissue engineering, stem cell research, nanotechnology and material science.
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
This book features the most cutting-edge work from the world’s leading laboratories in this field and provides practical methods for differentiating pluripotent stem cells into hematopoietic lineages in the blood system. Pluripotent stem cells have attracted major interest from a fast-growing and multidisciplinary community of researchers who are developing new techniques for the derivation and differentiation of these cells into specific cell lineages. These direct differentiation methods hold great promise for the translational applications of these cells. This book is an essential reference work for researchers at all levels in the fields of hematology and stem cell biology, as well as clinical practitioners in regenerative medicine.