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Since the first successful isolation and cultivation of human embryonic stem cells at the University of Wisconsin, Madison in 1998, there has been high levels of both interest and controversy in this area of research.
This book provides a concise overview of an exciting field, covering the characteristics of both human embryonic stem cells and pluripotent stem cells from other human cell lineages. The following chapters describe state-of-the-art differentiation and characterization of specific ectoderm, mesoderm and endoderm-derived lineages from human embryonic stem cells, emphasizing how these can be used to study human developmental mechanisms. A further chapter discusses genetic manipulation of human ES cells. The concluding section covers therapeutic applications of human ES cells, as well as addressing the ethical and legal issues that this research have raised.
The book outlines first the importance of Extra Cellular Matrix (ECM), which is a natural surface for most of cells. In the following chapters the influence of biological, chemical, mechanical, and physical properties of surfaces in micro and nano-scale on stem cell behavior are discussed including the mechanotransduction. Biomimetic and bioinspired approaches are highlighted for developing microenvironment of several tissues, and surface engineering applications are discussed in tissue engineering, regenerative medicine and different type of biomaterials in various chapters of the book.
This book brings together innovative methodologies and strategies adopted in the research and development of Advanced Surfaces in Stem Cell Research. Well-known worldwide researchers deliberate subjects including:
Extracellular matrix proteins for stem cell fate
The superficial mechanical and physical properties of matrix microenvironment as stem cell fate regulator
Effects of mechanotransduction on stem cell behavior
Modulation of stem cells behavior through bioactive surfaces
Influence of controlled micro and nanoengineered surfaces on stem cell fate
Nanostructured polymeric surfaces for stem cells
Laser surface modification techniques and stem cells applications
Plasma polymer deposition: a versatile tool for stem cell research
Application of bioreactor concept and modeling techniques in bone regeneration and augmentation treatments
Substrates and surfaces for control of pluripotent stem cell fate and function
Application of biopolymer-based, surface modified devices in transplant medicine and tissue engineering
Silk as a natural biopolymer for tissue engineering